Ultrasensitive detection of SARS-CoV-2 RNA and antigen using single-molecule optofluidic chip

Ultrasensitive detection of SARS-CoV-2 RNA and antigen using single-molecule optofluidic chip

Blog Article

Nucleic acids and proteins are the two most important target types used in molecular diagnostics.In many instances, simultaneous sensitive and accurate ah6789-001 detection of both biomarkers from the same sample would be desirable, but standard detection methods are highly optimized for one type and not cross-compatible.Here, we report the simultaneous multiplexed detection of SARS-CoV-2 RNAs and antigens with single molecule sensitivity.

Both analytes are isolated and labeled using a single bead-based solid-phase extraction protocol, followed by fluorescence detection on a multi-channel optofluidic waveguide chip.Direct amplification-free detection of both biomarkers from nasopharyngeal swab samples is demonstrated with single molecule detection sensitivity, opening the door for ultrasensitive dual-target analysis in infectious disease diagnosis, oncology, fg42 replica and other applications.